NoHistogram overlay controls allow you to normalize overlaid histograms and space them out by a continuous offset.
Overlay Controls
Figure 1 shows the histogram overlay controls available to you. Plot resolution is control by the dropdown in Figure 1.1. Larger numbers produce higher resolution. Whether to smooth the histogram or not is controlled by the checkbox shown in Figure 1.2. Normalization is set via the dropdown in Figure 1.3, and the offset distance is controlled by the slider in Figure 1.4.
Figure 1 Overlay Controls
| No. | Component |
|---|---|
| 1 | Resolution control |
| 2 | Smoothing check box |
| 3 | Normalization selector |
| 4 | Offset slider |
Normalization Options
You have four normalization options for FlowJo histograms:
- Auto: The Y-axis will be count and the range will be automatically set. This is a good option when you are creating a single histogram and all of the populations come from a common parent so that a difference in count is meaningful. This would also apply to a set of populations with different parents if the number of events in all parent populations has been made equal by sampling
- Modal
- Unit Area
- Manual