Released 11.05.2025
New Features
General
- v10 workspaces can be opened in v11 with some limitations.
- Population/parameter nodes can be imported, so this serves as a means of bringing the output of various algorithms into version 11.1.
- Keyboard accelerators have been added to allow for control of most functions via the keyboard
- Return to launcher for workspace navigation is now available.
- Warning provided to Mac users who have downloaded the wrong version for their processor
Metadata Manager
- Keyword import from .csv added to the Metadata Manager
Compensation Wizard
- Transform properties panel is now included in the Compensation Wizard
- Normalization of matrices now matches Chorus / Diva
- Compensation error messages are now more descriptive
Graph Window
- Color axis now uses an absolute scale
- Graphs are now high resolution.
- A control is provided to adjust resolution in the properties panel
- There is a preference for setting default resolution
- Backgating is now a graph gallery mode
- Downstream populations can be displayed in the graph gallery
- This features applies to all graph gallery modes; view by sample, view by population, Backgating, and ancestry
- Graphics can be exported as images or copy-pasted to the clipboard for use in other programs
Reports
- Additional plot control tools added
- Histogram overlay controls added
- High resolution graphics extends to Reports
Imaging
- FlowJo v11.1 can directly expand .CVW files into .TIFF images.
- There is a preference to control whether this happens automatically on detection of .CVW without .TIFF files
- Image wall performance improved
Bug Fixes
General
- Workbenches with FACSDiscover data can be exported to v10.
- FACSMelody files now load
- Bigfoot data now displayed with correct scale
- Engine no longer crashes if 0 event child populations are acted on
- Engine no longer crashes if corrupt data load is attempted
QC
- Gates under 'Good Events' are not deleted if QC is re-run.
Compensation
- Size gate modifications are maintained when switching between AutoSpill and gate based compensation.
- Graphs in the compensation Wizard no longer become empty when switching groups.
- Program no longer crashes when new workspace is opened directly after running compensation
- Custom transforms no longer reset when spillover matrix is edited.
Transformation
- Resetting the transformation no longer resets all scales to linear.
Graphs
- Arrow tool no longer draws rectangle gate on histograms
- Renaming quads no longer rearranges statistics
Tables
- Multiselect populations works with stat selection
Reports
- VCPs now display group name in legend instead of a file
- VCPs retain group association when multiple populatios are added to Reports
- Object snap back issue fixed
- Floating x-axis gate label removed
- Pasting text improved
- Histogram range gates removed from pseudo color plots
- Local preference changes will update reports
Imaging
- Image wall cell picker now scrolls to selected cell
Platforms
- tSNE performance improved
Preferences
- Preferences apply in the correct order or Workbench > User > Computer
- FACSDiscover default parameters correctly populate
- Preferences are confined to specific cytometer
Limitations and high priority Issues
File operation
- Bigfoot derived FCS files are displayed incorrectly with a reduced scale
- On windows, files won't load from folders with accent marks in the name
MetaData manager
- Parameters with the same $PnN but different $PnS will show an incorrect warning label indicating they are duplicates.
Unmixing
- In the unmixing wizard, NxN plots for BD FACSDiscover S8 data can have misleading labels
Hierarchy
- Editing the group criteria of a group created by dragging in a folder produces no effect.
Graph Window
- Quad gates will briefly move if desync'd and then double-clicked to view the population. Statistics are unaffected, this is purely a refresh issue.
- A zero event population shown on a histogram plot will turn the whole plot blue instead of white.
- When in VCP mode, population statistics are not updated after deleting a sample from a group. You will notice that hierarchies can display more events in the sub-population than the parent.
Charts
- Some extra padding can be introduced to the top of charts when p-values are enabled and the a Y-axis height has been manually.
- In numeric mode the comparison lines can sometimes overlap.
Reports
- Select All + Copy + Paste fails