FlowJoTM version 11 uses a new file format, the workbench, with a file extension of .flowjo.
Workspace files from FlowJoTM v10 are not compatible with the first release of FlowJo v11, and cannot be opened by or imported into FlowJo v11.0. Basic import of gates from saved v10 workspaces is currently in development and planned for a future release.
FlowJo v11 workbenches can be exported as v10 workspaces using the “Export as a FlowJo v10 (.wsp)” workspace action under the file menu (Figure 1).
Figure 1. Export to .wsp
Export as a FlowJo v10 (.wsp) will produce a new .wsp file that can be opened in v10, while maintaining the v11 workbench. The output will include a flattened group hierarchy (as version 10 does not support hierarchical groups), all groups, and gates. It does not include the tables, charts, or reports included in v11.