As Flow Cytometry experiments increase in complexity and in the number of parameters available in the exported FCS files, grouping desired parameters into different parameter sets may be of value. This allows for the quick access and utility of specified sets across the data analysis workflow, sparing time from scrolling within long lists of parameters.
To create a new parameter set, click on the Metadata Manager and select the Parameter Sets tab. The list on the left will show all available parameters, including their Fluorochrome Name ($PnN) and Stain Name ($PnS). Scatter and UnCompensated parameters can be toggled on/of (Figure 1.1).
The selected parameters can be then added to the right list of parameters (Figure 1.2). The set can be given a name and description (Figure 1.3, "Exclusion Parameters"). Multiple parameter sets can be added using the "+" button; existing lists can be duplicated or modified.
Figure 1. Creating a new parameter set within the Metadata Manager (here Exclusion Parameters)
Once a Parameter Set is created, it will be directly available in the rest of the functions within the workbench. The example below is using the user-defined parameter sets (Figure 2.1) on the Primary plot for quick access to the parameters to be displayed on the x- and y- axis as well as for batching per parameter (Figure 2.2).
Figure 2. Using the Exclusion parameters sets on the Primary plot to visualize parameters to use parameters on x- and y-axes and for batching by parameter