FlowJo Version 11.0.2 Release notes

Released 8.12.2025

New Features

  • BD Access Portal error messaging updated to accurately distinguish the lack of an internet connection from the lack of a license.

Improvements

  • Issue resulting in 'Repair and Restart' error message being displayed on Windows machines at install was addressed.

Limitations and high priority Issues

File operation

  • Return to launcher without saving, followed by quitting allows the user to quit without saving or a prompt
  • ID7000 data can return invalid statistics
  • Bigfoot derived FCS files are displayed incorrectly with a reduced scale
  • FACSMelody files will not load
  • Export to v10 fails for FACSDiscover data
  • Some workbenches will show the error "The file you tried to open is not supported" on attempt to open
  • On windows, files won't load from folders with accent marks in the name

Preferences

  • Preferences set on one cytometer can apply to other cytometers

Unmixing

  • In the unmixing wizard, NxN plots for BD FACSDiscover S8 data have misleading labels
  • The spillover matrix normalization approach differs from FACSDiva and FACSChorus.  Matrices will be proportionally the same, but will have different values.

Reports

  • Virtually concatenated data will display the incorrect group name in legends

Platforms

  • tSNE calculation slows down with repeated runs.
  • tSNE plots are less defined in v11 compared to v10

Image Wall

  • Large amounts of FACSDiscover data can freeze the image wall
  • Image wall filter settings reset on save/reopen a workbench