These data files can be read and analyzed by FlowJo; they are designed to demonstrate the many different types of data that FlowJo analyzes with ease. The data sets listed here can be read into FlowJo when it is running in "Demonstration Mode"--the free, fully-functional analysis mode, for which the only limitation is the ability to read only the data files provided by Tree Star, Inc. You do not need to purchase a user license, or even to apply for the free, 30-day serial number, in order to run Demonstration Mode. Simply download the program (or copy it from your CD) and begin analyzing!

If you want to see FlowJo work on your own data files, then you can apply for a free serial number (good for 30 days), that gives you the complete fully functional FlowJo with unrestricted operation.

We highly recommend that you start with one of our tutorials. Both the Basic and Advanced tutorials are guided tours, complete with example experiments and demo data for you to practice each step. Download the tutorial instructions and demo data from the tutorials page

Several sample data sets are below. Each is designed to highlight different aspects of features of the program. Each sample comes with a workspace file, data files, and a Read Me introduction.

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Stuffit Expander will permit your computer to decompress these files. It (or another program such as WinZip may already be on your machine.)

Compensation [Download Stuffit data - Zip data - 1 Mb]

This data set provides an introduction to performing compensation using FlowJo. For more information on FlowJo's Compensation platform, click here For Mac - For Windows . In addition, our Compensation Tech Note provides step-by-step instructions for performing compensation.

Cell Cycle [Download Stuffit data - Zip data - 42 Kb]

This simple example illustrates how to perform Cell Cycle analysis. This data set is comprised of five files, collected at various points after synchronization of a cell line. Tree Star thanks Tom Delohery for kindly providing this demonstration data set. For more information on FlowJo's Calibration platform, click here For Mac - For Windows

Proliferation [Download Stuffit data - Zip data - 315 Kb]

These samples were stained with CFSE, an intracellular dye used to measure cell proliferation, and then cultured for three days. With each cell division, the CFSE is divided between the two daughter cells. Therefore the amount of CFSE fluorescence shows the number of divisions any given cell has undergone. For more information on FlowJo's Proliferation platform, click here For Mac - For Windows

Kinetics [Download Stuffit data - Zip data - 9 Mb]

The example data set demonstrates several features of the sophisticated kinetics platform that FlowJo provides. Cells were loaded with Indo-1. Collection for 30 seconds was performed to establish the baseline calcium concentration (related to the ratio of the Indo-1 fluorescences); then anti-CD3 was added. Within about 1 minute cells begin to respond by fluxing calcium; the Indo-1 emission ratio changes substantially over time. For more information on FlowJo's Kinetics platform, click here For Mac - For Windows In addition, our Kinetics Tech Note provides step-by-step instructions for performing kinetic analyses.

Calibration [Download Stuffit data - Zip data - 460 Kb]

This example illustrates how you can calibrate a parameter based on a standardized set of beads. For more information on FlowJo's Calibration platform, click here For Mac - For Windows

Full Immunophenotyping [Download Stuffit data - Zip data - 40 Mb]

The Full Immunophenotyping data set serves as an example of how a large experiment is analyzed and how the analyses are organized by FlowJo. The analyses depend largely on the "Group"-based operations in FlowJo, where entire sets of samples (i.e., all tubes with the same stain combination) are gated identically. For more information on Groups, click here For Mac - For Windows

Complex Analysis [Download Stuffit data - Zip data - 3.3 Mb]

There is only a single data file in this example. It is a collection of 300,000 events of PBMC stained with 8 different antibody conjugates. The reagents used in this experiment are: Cascade Blue anti-CD3, FITC anti-CD11a, PE anti-TcR gd, Cy5PE anti-CD45RA, Cy7PE anti-CD62L, Texas Red anti-CD4, APC anti-CD57, and Cy7APC anti-CD8. The goal in this stain was to identify fine T cell subsets.

11 Color Demonstration [Download Stuffit data - Zip data - 50 Mb]

The PBMC sample in this collection was stained with 11 different antibodies: FITC anti-CD28, PE anti-CD49f, Cy5PE anti-CD11a, Alexa495 anti-CD62L, APC anti-CD45RO, Cy7APC anti-CD57, Cy7PE anti-CD4, Cascade Blue anti-CD45RA, Cascade Yellow anti-CD3, Cy5.5PE anti-CD27, and Cy5.5APC anti-CD8. The goal in this stain was to identify fine T cell subsets.

Large Data Files [Download Stuffit data - Zip data - 6.2 Mb]

A single PBMC sample was stained with antibodies to CD3, CD4, and CD8. The sample was collect four times, with increasing numbers of events: 1000, 10,000, 100,000, and 1 million. Using these data files, you can explore how different types of displays can be dramatically different even though essentially the same data is being viewed.